i.Mune™ Treg* is a quantitative test to determine the percentages and absolute** counts of human
TSDR+ regulatory T cells and CD3+ T cells in peripheral whole blood or in blood dried on filter paper.
Allows molecular quantification of1):
TSDR = Treg Specific De-methylated Region
The preferred method for accurate quantification of FOXP3+ Treg4)
Total CD4+ T cells show similar percentage of Treg by FOXP3 flow cytometry (A, left 10.4%) and by TSDR DNA-methylation analysis (B, left 12.7%).
Stimulated CD4+ T cells transiently express the FOXP3 protein (A, right 29.7%) but lack DNA de-methylation of the TSDR (B, right 1.4%). Hence, TSDR de-methylation analysis is more specific for Treg quantification than FOXP3 detection by flow cytometry1)4)
Quantification of Treg can be useful in**:
*i.Mune™Treg is for research use, only and has not been registered as an IVD product and has not been approved or cleared by a regulatory authority.
**The above clinical applications have been established using technologies currently being employed in clinical laboratory routine (e.g. flow cytometry). Epimune has demonstrated equivalence of its epigenetic immune cell quantification method to flow cytometry (in whole blood) and the concordance of epigenetic immune cell quantification between whole blood and dried blood spots1)