i.Mune™ TBNK [CE] a quantitative in vitro test to determine the percentages and absolute counts of human lymphocyte subsets in liquid venous whole blood.
i.Mune™ TBNK [CE] allows the epigenetic quantification of:
i.Mune™ TBNK [CE] is comprised of the three (3) separate kits:
In addition, we'll provide a Data Analysis Tool (MS-Excel based)
Quantification of T-/B- and NK lymphocytes can be useful for*:
Please inquire to obtain a customized proposal:
*The above clinical applications have been established using technologies currently being employed in clinical laboratory routine (e.g. flow cytometry).
Performance characteristics of i.Mune™ TBNK [CE] were determined according to CLSI guidelines.
|Repeatability (single-site)||CV < 12,5%|
|Within Laboratory Precision (single-site)||
CV < 25%
CV < 25%
|Within Laboratory Precision (multi-site)||
CV < 25%
10 - 40 µl venous whole blood
|Limit of Quantification||
< 100 copies
No interference with bilirubin (0,4 mg/ml),
Clinical performance of i.Mune™ TBNK [CE] was evaluated according to DIN EN 13612. For this 113 liquid venous whole blood samples from self-declared healthy donors were prospectively collected in K2-EDTA blood collection tubes. Epigenetic data of 112 test samples analysed with i.Mune™ TBNK [CE] were compared to flow cytometry data using the Spearman correlation coefficient (Spearman r) for each assay (% and cells/μl).
|Assay||Spearman r||p value|
|CD3 % of Leukocytes||0.85||<0.0001|
|CD4 % of Leukocytes||0.76||<0.0001|
|CD8 % of Leukocytes||0.53||<0.0001|
|BLC % of Leukocytes||0.86||<0.0001|
|NKC % of Leukocytes||0.78||<0.0001|
Comparison i.Mune™ TBNK [CE] with flow cytometry
(n=112 self declared healthy adult donors)
r = 0.90
r = 0.96