i.Mune™ PID* is a quantitative test to determine the percentages and absolute** counts of human
CD3+, CD4+, CD8+ T-, B-, NK cells, TSDR+ regulatory T cells, neutrophils and monocytes in peripheral whole blood or in blood dried on filter paper.
Allows molecular quantification of:
TSDR = Treg Specific De-methylated Region
Results are comparable to flow cytometry
Comparison of immune cell quantification by flow cytometry and epigenetic qPCR in whole blood samples of healthy donors (n=25)
Top: Relative quantification (% cells); bottom: Absolute quantification (cells/μl)1)
Can be useful for**:
Primary Immunodeficiency diagnostic tests were recently included in WHO's Essential Diagnostics List (EDL), published 09 July 2019
*i.Mune™ PID is for research use, only and has not been registered as an IVD product and has not been approved or cleared by a regulatory authority.
**The above clinical applications have been established using technologies currently being employed in clinical laboratory routine (e.g. flow cytometry). Epimune has demonstrated equivalence of its epigenetic immune cell quantification method to flow cytometry (in whole blood) and the concordance of epigenetic immune cell quantification between whole blood and dried blood spots1)