i.Mune™ Treg* is a quantitative test to determine the percentages and absolute** counts of human
TSDR+ regulatory T cells and CD3+ T cells in peripheral whole blood or in blood dried on filter paper.
Allows molecular quantification of1):
TSDR = Treg Specific De-methylated Region
The preferred method for accurate quantification of FOXP3+ Treg4)
Total CD4+ T cells show similar percentage of Treg by FOXP3 flow cytometry (A, left 10.4%) and by TSDR DNA-methylation analysis (B, left 12.7%).
Stimulated CD4+ T cells transiently express the FOXP3 protein (A, right 29.7%) but lack DNA de-methylation of the TSDR (B, right 1.4%). Hence, TSDR de-methylation analysis is more specific for Treg quantification than FOXP3 detection by flow cytometry1)4)
Quantification of Treg can be useful in**:
*i.Mune™Treg is for research use, only and has not been registered as an IVD product and has not been approved or cleared by a regulatory authority.