i.Mune® B [RUO]

(not available in the United States of America)


i.Mune B [RUO] is a quantitative in vitro test to determine the percentages and absolute counts of human  B lymphocytes in liquid venous whole blood and capillary whole blood specimens dried on filter paper (Dried Blood Spot; DBS).

Please note - i.Mune B is for research use only. Not for diagnostic purposes.

Benefits

i.Mune B [RUO] enables the epigenetic quantification of:

  • B lymphocytes (CD19+)

Throughput:

  • Kit is designed to test 8 or more samples, plus Controls, Standard, in one PCR-run (96-well)

Contents

i.Mune B is comprised of two (2) separate kits:

  • i.Mune Prep Kit (for preparation and bisulfite-conversion of DNA from fresh, frozen or dried blood samples)
  • i.Mune B PCR Kit (for PCR-amplification of bisulfite-converted DNA, Positive and Negative Controls, Standard and Quantifier)
  • Data Analysis Tool (MS-Excel based)

Sample Requirements


  • Capillary whole blood dried on filter paper (DBS) (Whatman 903 Protein Saver Cards, Ahlstrom-Munksjö TFN Cards, Capitainer Cards)
    • Samples can be stored at room temperature (15°C to 30°C) or frozen (-30°C to -15°C) for up to 12 weeks
    • DBS sample stability has been demonstrated at 55°C and 65-78% humidity for up to 7 days 

OR

  • 40 µl of liquid venous whole blood collected in K2EDTA blood collection tube
    • Samples can be stored at room temperature (15°C to 30°C) for up to 24 h or frozen (-30°C to -15°C) for up to 12 weeks

Applications

Quantification of  B lymphocytes can be useful for:

  • Monitoring of patients in B-cell depleting therapies 1)

Please note - i.Mune B is for research use only. Not for diagnostic purposes.

References

  1. Tallantyre et al, Home-sampling of B cells using quantitative dried blood spots to enable tailored therapeutic re-dosing of anti-CD20 therapies, MSJ 2025

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