i.Mune® CD4/CD8 [RUO]


i.Mune CD4 [RUO] is a quantitative in vitro test to determine the percentages and absolute counts of human CD4+ T lymphocytes in liquid venous whole blood and capillary whole blood specimens dried on filter paper (Dried Blood Spot; DBS).

Please note - i.Mune CD4 is for research use only. Not for diagnostic purposes.

Benefits

i.Mune CD4 [RUO] enables the epigenetic quantification of:

  • Helper T lymphocytes (CD3+CD4+)

Throughput:

  • Process up to 68 samples, plus Controls, Standard, in one PCR-run (96-well)

Contents

i.Mune CD4 is comprised of the three (3) separate kits:

  • i.Mune Prep (for preparation and bisulfite-conversion of DNA from fresh, frozen or dried blood samples)
  • i.Mune CD4 Amp (for PCR-amplification of bisulfite-converted DNA)
  • i.Mune CD4 Check (Positive and Negative Controls, Standard)
  • Data Analysis Tool (MS-Excel based)

Sample Requirements


  • Capillary whole blood dried on filter paper (DBS)
    • Samples can be stored at room temperature (15°C to 30°C) or frozen (-30°C to -15°C) for up to 12 weeks
    • DBS sample stability has been demonstrated at 55°C and 65-78% humidity for up to 7 days 

OR

  • 40 µl of liquid venous whole blood collected in K2EDTA blood collection tube
    • Samples can be stored at room temperature (15°C to 30°C) for up to 24 h or frozen (-30°C to -15°C) for up to 12 weeks

Applications

Quantification of  CD4+ lymphocytes can be useful for*:

  • Monitoring of HIV-positive patients 1),2)

Please note - i.Mune CD4 is for research use only. Not for diagnostic purposes.

97 blood samples from HIV positive subjects (liquid and dried) were analyzed using flow cytometry (liquid blood) and epigenetic qPCR (liquid and dried), respectively. The data show excellent correlation between both testing methods and between liquid and dried blood 1).

Please inquire for a customized proposal:

References

  1. Baron U. et al. Epigenetic immune cell counting in human blood samples for immunodiagnostics. Sci Transl Med. 2018 Aug 1; 10 (452)
  2. Ford N, et al. The evolving role of CD4 cell counts in HIV care. Curr Opin HIV AIDS. 2017 Mar;12(2):123-128
  3. https://www.gesundheit.gv.at/labor/laborwerte/immunsystem/cd43-cd83-ratio.html