i.Mune® CD4 [RUO]


i.Mune CD4 [RUO] is a quantitative in vitro test to determine the percentages and absolute counts of human CD4+  T lymphocytes in liquid venous whole blood and capillary whole blood specimens dried on filter paper (Dried Blood Spot; DBS).

Please note - i.Mune CD4 is for research use only. Not for diagnostic purposes.

Benefits

i.Mune CD4 [RUO] enables the epigenetic quantification of:

  • Helper T lymphocytes (CD3+CD4+)

Throughput:

  • Analyze up to 68 samples, Controls, Standard, in one PCR run (96-well)

Contents

i.Mune CD4 is comprised of the three (3) separate kits:

  • i.Mune Prep (for preparation and bisulfite-conversion of DNA from fresh, frozen or dried blood samples)
  • i.Mune CD4 Amp (for PCR-amplification of bisulfite-converted DNA)
  • i.Mune CD4 Check (Positive and Negative Controls, Standard)
  • Data Analysis Tool (MS-Excel based)

Sample Requirements


  • Capillary whole blood dried on filter paper (DBS) (Whatman 903, Munktell TFN, Capitainer)
    • Samples can be stored at room temperature (15°C to 30°C) or frozen (-30°C to -15°C) for up to 12 weeks
    • DBS sample stability has been demonstrated at 55°C and 65-78% humidity for up to 7 days 

OR

  • 40 µl of liquid venous whole blood collected in K2EDTA blood collection tube
    • Samples can be stored at room temperature (15°C to 30°C) for up to 24 h or frozen (-30°C to -15°C) for up to 12 weeks

Applications

Please note - i.Mune CD4 is for research use only. Not for diagnostic purposes.

Please inquire for a customized proposal:

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References

  1. Baron U. et al. Epigenetic immune cell counting in human blood samples for immunodiagnostics. Sci Transl Med. 2018 Aug 1; 10 (452)
  2. Ford N, et al. The evolving role of CD4 cell counts in HIV care. Curr Opin HIV AIDS. 2017 Mar;12(2):123-128
  3. https://www.gesundheit.gv.at/labor/laborwerte/immunsystem/cd43-cd83-ratio.html

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