i.Mune CD4/CD8 [RUO] is a quantitative in vitro test to determine the percentages and absolute counts* of human CD4+ and CD8+ T lymphocytes in liquid venous whole blood and capillary whole blood specimens dried on filter paper (Dried Blood Spot; DBS) or Plasma Separation Cards (PSC).
(*absolute quantification in liquid blood (fresh or frozen), only)
Please note - i.Mune CD4/CD8 is for research use only. Not for diagnostic purposes.
i.Mune CD4/CD8 [RUO] enables the epigenetic quantification of:
Throughput:
i.Mune CD4/CD8 is comprised of the three (3) separate kits:
OR
Quantification of CD4+ and CD8+ lymphocytes can be useful for*:
*The above clinical applications have been established using technologies currently being employed in clinical laboratory routine (e.g. flow cytometry).
Please note - i.Mune CD4/CD8 is for research use only. Not for diagnostic purposes.
Comparison i.Mune CD4/CD8 [RUO] with flow cytometry
247 frozen venous whole blood samples from patients with various different primary and secondary immunodeficiencies were analyzed using i.Mune CD4/CD8 and compared to flow cytometry data obtained at time of blood sampling. Correlation was determined using the Spearman correlation coefficient (Spearman r) for each assay (cells/µl and % leukocytes).
Epigenetic quantification of CD4+ and CD8+ T-cells in Dried Blood Spots (DBS)
97 blood samples from HIV positive subjects (liquid and dried) were analyzed using flow cytometry (liquid blood) and epigenetic qPCR (liquid and dried), respectively. The data show excellent correlation between both testing methods and between liquid and dried blood 1).
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