i.Mune® CD4/CD8 [RUO]
i.Mune CD4/CD8 [RUO] is a quantitative in vitro test to determine the percentages and absolute counts* of human CD4+ and CD8+ T lymphocytes in liquid venous whole blood and capillary whole blood specimens dried on filter paper (Dried Blood Spot; DBS) or Plasma Separation Cards (PSC).
(*absolute quantification in liquid blood (fresh or frozen), only)
Please note - i.Mune CD4/CD8 is for research use only. Not for diagnostic purposes.
Benefits
i.Mune CD4/CD8 [RUO] enables the epigenetic quantification of:
- Helper T lymphocytes (CD3+CD4+)
- Cytotoxic T lymphocytes (CD3+CD8+)
Throughput:
- Up to 20 samples per PCR-run (96-well)
Contents
i.Mune CD4/CD8 is comprised of the three (3) separate kits:
- i.Mune Prep (for preparation and bisulfite-conversion of DNA from fresh, frozen or dried blood samples)
- i.Mune CD4/CD8 Amp (for PCR-amplification of bisulfite-converted DNA)
- i.Mune CD4/CD8 Check (Positive and negative controls, standards)
- Data Analysis Tool (MS-Excel based)
Sample Requirements
- Capillary whole blood dried on filter paper (DBS) or Plasma Separation Cards (PSC)
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- Samples can be stored at room temperature (15°C to 30°C) or frozen (-30°C to -15°C) for up to 12 weeks
- DBS sample stability has been demonstrated at 55°C and 65-78% humidity for up to 7 days
OR
- 40 µl of liquid venous whole blood collected in K2EDTA blood collection tube
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- Samples can be stored at room temperature (15°C to 30°C) for up to 24 h or frozen (-30°C to -15°C) for up to 12 weeks
Applications
Please note - i.Mune CD4/CD8 is for research use only. Not for diagnostic purposes.
Please inquire for a customized proposal:
References
- Baron U. et al. Epigenetic immune cell counting in human blood samples for immunodiagnostics. Sci Transl Med. 2018 Aug 1; 10 (452)
- Ford N, et al. The evolving role of CD4 cell counts in HIV care. Curr Opin HIV AIDS. 2017 Mar;12(2):123-128
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https://www.gesundheit.gv.at/labor/laborwerte/immunsystem/cd43-cd83-ratio.html